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dc.contributor.authorPrasad, S-
dc.contributor.authorYadav, V R-
dc.contributor.authorChitra, S-
dc.contributor.authorReuter, S-
dc.contributor.authorHema, P S-
dc.contributor.authorMangalam S Nair-
dc.contributor.authorChaturvedi, M M-
dc.contributor.authorAggarwal, B B-
dc.date.accessioned2014-03-24T09:10:38Z-
dc.date.available2014-03-24T09:10:38Z-
dc.date.issued2010-
dc.identifier.citationJournal of Biological Chemistry 285(35):26987-26997;27 Aug2010en_US
dc.identifier.issn0021-9258-
dc.identifier.urihttp://ir.niist.res.in:8080/jspui/handle/123456789/1196-
dc.description.abstractCrotepoxide (a substituted cyclohexane diepoxide), isolated from Kaempferia pulchra (peacock ginger), although linked to antitumor and anti-inflammatory activities, the mechanism by which it exhibits these activities, is not yet understood. Because nuclear factor kappa B (NF-kappa B) plays a critical role in these signaling pathways, we investigated the effects of crotepoxide on NF-kappa B-mediated cellular responses in human cancer cells. We found that crotepoxide potentiated tumor necrosis factor (TNF), and chemotherapeutic agents induced apoptosis and inhibited the expression of NF-kappa B-regulated gene products involved in anti-apoptosis (Bcl-2, Bcl-xL, IAP1,(2) MCl-1, survivin, and TRAF1), apoptosis (Bax, Bid), inflammation (COX-2), proliferation (cyclin D1 and c-myc), invasion (ICAM-1 and MMP-9), and angiogenesis (VEGF). We also found that crotepoxide inhibited both inducible and constitutive NF-kappa B activation. Crotepoxide inhibition of NF-kappa B was not inducer-specific; it inhibited NF-kappa B activation induced by TNF, phorbol 12-myristate 13-acetate, lipopolysaccharide, and cigarette smoke. Crotepoxide suppression of NF-kappa B was not cell type-specific because NF-kappa B activation was inhibited in myeloid, leukemia, and epithelial cells. Furthermore, we found that crotepoxide inhibited TAK1 activation, which led to suppression of I kappa B alpha kinase, abrogation of I kappa B alpha phosphorylation and degradation, nuclear translocation of p65, and suppression of NF-kappa B-dependent reporter gene expression. Overall, our results indicate that crotepoxide sensitizes tumor cells to cytokines and chemotherapeutic agents through inhibition of NF-kappa B and NF-kappa B-regulated gene products, and this may provide the molecular basis for crotepoxide ability to suppress inflammation and carcinogenesis.en_US
dc.language.isoenen_US
dc.publisherAmerican Society for Biochemistry Molecular Biology Incen_US
dc.subjectGene-productsen_US
dc.subjectTyrosine kinaseen_US
dc.subjectProstate-canceren_US
dc.subjectCroton macrostachysen_US
dc.subjectKaempferia-rotundaen_US
dc.subjectTranscription factoren_US
dc.subjectCyclohexane diepoxideen_US
dc.subjectNecrosis-factoren_US
dc.subjectAlpha kinase activationen_US
dc.titleCrotepoxide chemosensitizes tumor cells through inhibition of expression of proliferation, invasion, and angiogenic proteins linked to proinflammatory pathwayen_US
dc.typeArticleen_US
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