Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/1497
Full metadata record
DC FieldValueLanguage
dc.contributor.authorNisha Rani-
dc.contributor.authorBeena Joy-
dc.contributor.authorEmilia Abraham, T-
dc.date.accessioned2014-06-09T11:49:15Z-
dc.date.available2014-06-09T11:49:15Z-
dc.date.issued2007-
dc.identifier.citationPharmaceutical Biology 45(1):48–53;2007en_US
dc.identifier.urihttp://ir.niist.res.in:8080/jspui/handle/123456789/1497-
dc.description.abstract3,4-Dihydroxy L-phenylalanine (L-DOPA) is considered a potent drug for the treatment of Parkinson disease, a neurologic disorder. Enantiomerically pure L-DOPA is produced from L-tyrosine in a single-step biotransformation process using callus cultures of the plant Portulaca grandiflora Hook (Portulacaceae). Callus cultures were induced in MS medium provided with growth regulators such as benzylaminopurine (BA; 1.5 mg L 1) and 2,4-dichlorophenoxyacetic acid (2,4-D; 0.1 mg L 1)and were found to be an excellent source of tyrosinase,which in turn was used for the biotransformation of Ltyrosine into L-DOPA. A culture time of 20–25 days was found to be optimum for biomass production,and the tyrosinase activity in the medium was found to be 2.19U=mL. Optimization of L-DOPA production was carried out by varying the concentration of BA and 2,4-D. In view of the fact that the enzyme tyrosinase has a dicopper catalytic site, the concentration of Cu2þ was manipulated in the media to study its impact on biotransformation rate. The L-DOPA was purified by column chromatography and the analysis was done by TLC, HPLC, FT IR, and MS. The optimized production of L-DOPA, 48.8 mg L 1 h 1, is one of the highest values recorded in the literature.en_US
dc.language.isoenen_US
dc.publisherInforma Healthcareen_US
dc.subjectBenzylaminopurineen_US
dc.subjectBiotransformationen_US
dc.subjectCallusen_US
dc.subjectCell suspensionen_US
dc.subject2,4-dichlorophenoxyacetic aciden_US
dc.subjectL-DOPAen_US
dc.subjectL-tyrosineen_US
dc.subjectPortulacaceaeen_US
dc.subjectTyrosinaseen_US
dc.subjectMSen_US
dc.titleCell suspension cultures of portulaca grandiflora as potent catalysts for biotransformation of L-tyrosine into L-dopa,an anti-parkinson’s drugen_US
dc.typeArticleen_US
niist.citation-
Appears in Collections:2007

Files in This Item:
File Description SizeFormat 
2007_00203.pdf
  Restricted Access
384.35 kBAdobe PDFView/Open Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.