Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/1547
Title: PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition
Authors: Fernandez, M R
Casabona, M G
Anupama, V N
Krishnakumar, B
Curutchet, G A
Bernik, D L
Keywords: SEM
CSLM
PDMS
Biofilm
Bed material
Acrylamide
Poly(dimethylsiloxane) microfluidic devices
Issue Date: 2010
Publisher: Elsevier
Citation: Colloids and Surfaces B-Biointerfaces 81(1):289-296;01 Nov 2010
Abstract: The objective of this work is to test the performance of new synthetic polydimethylsiloxane (PDMS)-based bed particles acting as carriers for bacteria biofilms. The particles obtained have a highly interconnected porous structure which offers a large surface adsorption area to the bacteria. In addition, PDMS materials can be cross-linked by copolymerization with other polymers. In the present work we have chosen two hydrophilic polymers: xanthan gum polysaccharide and tetraethoxysilane (TEOS). This versatile composition helps to modulate the interfacial hydrophobic/hydrophilic balance at the particle surface level and the roughness topology and pore size distribution, as revealed by scanning electron microscopy. Biofilm formation of a consortium isolated from a tannery effluent enriched in Sulphate Reducing Bacteria (SRB), and pure Acidithiobacillus ferrooxidans (AF) strains were assayed in three different bed particles synthesized with pure PDMS, PDMS-xanthan gum and PDMS-TEOS hybrids. Bacterial viability assays using confocal laser scanning fluorescence microscopy indicate that inclusion of hydrophilic groups on particle's surface significantly improves both cell adhesion and viability.
URI: http://ir.niist.res.in:8080/jspui/handle/123456789/1547
ISSN: 0927-7765
Appears in Collections:2010

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