Plant growth-promoting activity in newly isolated Bacillus thioparus (NII-0902) from Western ghat forest, India

Abstract

A Gram-positive rod-shaped bacterium isolated on nutrient agar plates incubated at 28 +/- A 2A degrees C. The identity of the bacterium was confirmed by sequencing of the 16S rRNA gene and it reveals that it shares highest similarity with Bacillus thioparus CECT 7196(T) (99.08%). It was capable of growing at temperatures ranging from 4 to 40A degrees C, but optimum growth was observed at 28 +/- A 2A degrees C. Strain NII-0902 is endowed with multiple plant growth promotion attributes such as phosphate solubilization, Indole acetic acid (IAA), siderophore and HCN production, which were expressed differentially at sub-optimal temperatures (5-40A degrees C). It was able to solubilize phosphate (17.7 mu g ml(-1)), and produce IAA (139.7 mu g ml(-1)) at 28 +/- A 2A degrees C. Qualitative detection of siderophore production and HCN were also observed. At 5A degrees C it was found to express all the plant growth promotion attributes except HCN production. The ability to colonize roots is a sine qua non condition for a rhizobacteria to be considered a true plant growth-promoting rhizobacteria (PGPR). Bacillus sp. NII-0902 has a potential ability to colonize roots visualized by transparency, bacterial growth (turbid, milky and narrow zone) along and around roots and truly supported by scanning electron micrograph. Hence, it is proposed that, Bacillus thioparus sp. NII-0902 could be deployed as an inoculant to attain the desired results of bacterization.