Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/1658
Title: A lysosome-targeted drug delivery system based on sorbitol backbone towards efficient cancer therapy
Authors: Santhi, M
Vandana, S
Jyothi, B N
Raghu, K G
Maiti, K K
Keywords: Lysosome-targeted drug delivery system
Cancer therapy
Tetrapeptide GLPG
Lysosomal cysteine protease
Issue Date: 2014
Publisher: Royal Society of Chemistry
Citation: Organic & Biomolecular Chemistry 12(34):6564-6569;6 Aug 2014
Abstract: A straightforward synthetic approach was adopted for the construction of a lysosome-targeted drug delivery system (TDDS) using sorbitol scaffold (Sor) linked to octa-guanidine and tetrapeptide GLPG, a peptide substrate of lysosomal cysteine protease, cathepsin B. The main objective was to efficiently deliver the potential anticancer drug, doxorubicin to the target sites, thereby minimizing dose-limiting toxicity. Three TDDS vectors were synthesized viz., DDS1: Sor-GLPG-Fl, DDS2: Sor-Fl (control) and DDS3: Sor-GLPGC-SMCC-Dox. Dox release from DDS3 in the presence of cathepsin B was studied by kinetics measurement based on the fluorescent property of Dox. The cytotoxicity of DDS1 was assessed and found to be non-toxic. Cellular internalization and colocalization studies of all the 3 systems were carried out by flow cytometry and confocal microscopy utilizing cathepsin B-expressing HeLa cells. DDS1 and DDS3 revealed significant localization within the lysosomes, in contrast to DDS2 (control). The doxorubicin-conjugated carrier, DDS3, demonstrated significant cytotoxic effect when compared to free Dox by MTT assay and also by flow cytometric analysis. The targeted approach with DDS3 is expected to be promising, because it is indicated to be advantageous over free Dox, which possesses dose-limiting toxicity, posing risk of injury to normal tissues.
ISSN: 1477-0539
Appears in Collections:2014

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