Parmotrema tinctorum exhibits antioxidant, antiglycation and inhibitory activities against aldose reductase and carbohydrate digestive enzymes: An in vitro study

Abstract

This study evaluated the inhibitory potential of ethyl acetate extract of Parmotrema tinctorum (PTEE), an edible lichen, against aldose reductase (AR) and carbohydrate digestive enzymes such as alpha-glucosidase and alpha-amylase. It was also screened for antioxidant activities by using DPPH, ABTS, superoxide and hydroxyl radical-scavenging assays. PTEE exhibited alpha-glucosidase, alpha-amylase and AR inhibition along with significant antiglycation potential with an estimated IC50 value of 58.45±1.24, 587.74±3.27, 139.28±2.6 and 285.78±1.287mug/mL, respectively. Antioxidant activity of PTEE against DPPH (IC50 396.83±2.98mug/mL), ABTS (151.34±1.79mug/mL), superoxide (30.29±1.17mug/mL) and hydroxyl (35.42±1.22mug/mL) radicals suggests the antioxidant potential of P. tinctorum. Significant antioxidant activity and inhibitory potential against carbohydrate digestive enzymes and AR suggest that P. tinctorum can be developed as functional food/nutraceuticals for diabetes after detailed study.