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dc.contributor.authorSinghania, R R-
dc.contributor.authorRajeev K Sukumaran-
dc.contributor.authorPandey, A-
dc.date.accessioned2015-08-06T08:56:30Z-
dc.date.available2015-08-06T08:56:30Z-
dc.date.issued2007-
dc.identifier.citationApplied Biochemistry and Biotechnology142(1):60-70;Jul 2007en_US
dc.identifier.issn0273-2289-
dc.identifier.urihttp://ir.niist.res.in:8080/jspui/handle/123456789/1914-
dc.description.abstractThe major constraint in the enzymatic saccharification of biomass for ethanol production is the cost of cellulase enzymes. Production cost of cellulases may be brought down by multifaceted approaches which includes the use of cheap lignocellulosic substrates for fermentation production of the enzyme, and the use of cost efficient fermentation strategies like solid state fermentation (SSF). The current study investigated the production of cellulase by Trichoderma reesei RUT C30 on wheat bran under SSF. Process parameters important in cellulase production were identified by a Plackett and Burman design and the parameters with significant effects on enzyme production were optimized for maximal yield using a central composite rotary design (CCD). Higher initial moisture content of the medium had a negative effect on production whereas incubation temperature influenced cellulase production positively in the tested range. Optimization of the levels of incubation temperature and initial moisture content of the medium resulted in a 6.2 fold increase in production from 0.605 to 3.8 U/gds of cellulase. The optimal combination of moisture and temperature was found to be 37.56% and 30 degrees C, respectively, for maximal cellulase production by the fungus on wheat bran.en_US
dc.language.isoenen_US
dc.publisherHumana Pressen_US
dc.subjectCellulaseen_US
dc.subjectTrichoderma reeseien_US
dc.subjectSolid state fermentationen_US
dc.subjectWheat branen_US
dc.subjectPlackett and burmanen_US
dc.subjectCentral composite designen_US
dc.subjectSubstrate fermentationen_US
dc.subjectAspergillus-nigeren_US
dc.subjectEthanolen_US
dc.titleImproved cellulase production by Trichoderma reesei RUT C30 under SSF through process optimizationen_US
dc.typeArticleen_US
niist.citation-
Appears in Collections:2007

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