Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/2070
Title: Stability of glucose oxidase activity of Aspergillus niger spores produced by solid-state fermentation and their role as biocatalysts in bioconversion reaction
Authors: Sumitra, R
Fontanille, P
Pandey, A
Larroche, C
Keywords: Aspergillus niger
Calcium gluconate
Fungal spores
Gluconic acid
Glucose oxidase
Sodium gluconate
Acid production
Sporulation
Issue Date: 2008
Publisher: Faculty Food Technology Biotechnology
Citation: Food Technology and Biotechnology 46(2):190-194;Apr-Jun 2008
Abstract: The aim of this work is to demonstrate the role of conidial spores as a reservoir of glucose oxidase and their stability as a biocatalyst in the bioconversion reaction for the production of gluconic acid. Solid-state fermentation (SSF) was carried out in fixed-bed column bioreactor for the production of Aspergillus niger spores. Growth parameters, sporulation and kinetics of gluconic acid production were analysed at different time intervals during the course of SSE Spores of different age (48-216 h) were used as biocatalysts in the bioconversion reaction. Spores harvested at a later period of SSF (196 h) produced high titres of gluconic acid (30 g/L) in the bioconversion medium when compared to the spores harvested at early (48 h) stages of SSF (2.2 g/L). Spores (harvested at 200 h and stored in freezer for 91 days) exhibited the same glucose oxidase activity and served as an active and stable catalyst when compared to the fresh spores, showing that aging (storage) did not affect enzymes present in the spores, which suggested that the spores acted as an efficient enzyme reservoir. Yields close to 93 % were obtained with 98 g/L of gluconic acid production, corresponding to an average productivity of 1.7 g/(L.h). The stability of the enzyme in the spores and the ability of conidia to be stored for a long time without the loss of activity add specific advantage to the bioconversion process.
URI: http://ir.niist.res.in:8080/jspui/handle/123456789/2070
ISSN: 1330-9862
Appears in Collections:2008

Files in This Item:
File Description SizeFormat 
2008_0135.PDF
  Restricted Access
126.53 kBAdobe PDFView/Open Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.