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dc.contributor.authorNishanth Kumar, S-
dc.contributor.authorBala Nambisan-
dc.contributor.authorSundaresan, A-
dc.contributor.authorMohandas, C-
dc.contributor.authorAnto, R J-
dc.date.accessioned2016-02-11T10:47:56Z-
dc.date.available2016-02-11T10:47:56Z-
dc.date.issued2014-03-
dc.identifier.citationAnnals of Microbiology 64(1):209-218,Mar 2014en_US
dc.identifier.urihttp://ir.niist.res.in:8080/jspui/handle/123456789/2240-
dc.description.abstractThe cell-free culture filtrate of Bacillus cereus associated with an entomopathogenic nematode, Rhabditis (Oscheius) sp.,exhibited strong antimicrobial activity. The ethyl acetate extract of the bacterial culture filtrate was purified by silica gel column chromatography to obtain six bioactive compounds. The structure and absolute stereochemistry of these compounds were determined based on extensive spectroscopic analyses (LCMS, FABMS, 1H NMR, 13C NMR, 1H −1H COSY, 1H −13C HMBC) and Marfey’s method. The compounds were identified as cyclo(D-Pro-D-Leu), cyclo(L-Pro-D-Met), cyclo (L-Pro-DPhe),cyclo (L-Pro-L-Val), 3,5-dihydroxy-4-ethyl-trans-stilbene,and 3,5-dihydroxy-4-isopropylstilbene, respectively.Compounds recorded antibacterial activity against all four tested bacteria strains of Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.3,5-dihydroxy-4-isopropylstilbene recorded activity only against Gram-positive bacteria while cyclo(L-Pro-L-Val)recorded no antibacterial activity. Best antibacterial activity was recorded by 3,5-dihydroxy-4-ethyl-trans-stilbene(4 μg/ml) against Escherichia coli. The six compounds recorded significant antifungal activities against five fungal strains tested (Aspergillus flavus, Candida albicans,Fusarium oxysporum, Rhizoctonia solani and Penicillium expansum) and they were more effective than bavistin, thestandard fungicide. The activity of cyclo(D-Pro-D-Leu),cyclo(L-Pro-D-Met), 3,5-dihydroxy-4-ethyl-trans-stilbene, and 3,5-dihydroxy-4-isopropylstilbene against Candida albicans was better than amphotericin B. To the best of our knowledge, this is the first report of antifungal activity of the bioactive compounds against the plant pathogenic fungi Fusarium oxysporum, Rhizoctonia solani, and Penicillium expansum. We conclude that the Bacillus cereus strain associated with entomopathogenic nematode is a promising source of natural bioactive secondary metabolites which may receive great benefit as potential sources of new drugs in the agricultural and pharmacological industry.en_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.subjectBacillus cereus; Secondary metabolite; Purification; Antimicrobialen_US
dc.titleIsolation and identification of antimicrobial secondary metabolites from Bacillus cereus associated with a rhabditid entomopathogenic nematodeen_US
dc.typeArticleen_US
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