Please use this identifier to cite or link to this item: http://ir.niist.res.in:8080/jspui/handle/123456789/2380
Title: Characterization of three depside compounds from a Western Ghat lichenParmelia erumpens Kurok with special reference to antimicrobial and anticancer activity
Authors: Aravind, S R
Sreelekha, T T
Dileep Kumar, B S
Nishanth Kumar, S
Mohandas, C
Keywords: antimicrobial
Issue Date: 1-Aug-2014
Publisher: RSC Advances
Citation: Royal Society of Chemistry 4:34632–34643
Abstract: The aim of this study was to investigate the major chemical compositions of the chloroform extract of lichen Parmelia erumpens from Western Ghats, Kerala, India and its antimicrobial and anticancer activities. Chloroform extract was purified by silica gel column chromatography to obtain three major compounds and their chemical structures were characterized by 1H-NMR, 13 C-NMR, UV and HR-MS spectroscopic methods as atranorin (1), (+)-usnic acid (2) and 2-hydroxy-4-methoxy-3,6-dimethylbenzoic acid (3). The minimal inhibitory concentration (MIC) by the broth micro dilution and agar disc diffusion methods was used to record the antimicrobial activity. Out of three compounds tested, 2-hydroxy-4-methoxy-3,6-dimethylbenzoic acid recorded excellent antimicrobial activity especially against medically important bacteria and fungi and the MIC values ranged from 0.06 to 4mg ml 1 against test bacteria and 0.12 to 16mgml 1 against test fungi. The best MIC of 0.06mgml 1 by 2-hydroxy-4-methoxy-3,6-dimethylbenzoic acid was recorded againstVibrio cholera, a human pathogenic bacterium responsible for causing life threatening diseases like profuse watery diarrhea. Anti cancer activity was initially screened by MTT assay in A549, B16F10, Caski and HepG2 cell lines. MTT assay results showed that the growth of cancer cells was suppressed by 2-hydroxy-4-methoxy-3,6-dimethylbenzoic acid in both dose- and time-dependent manners. A549, B16F10 and Caski cells treated with 2-hydroxy-4-methoxy-3,6-dimethylbenzoic acid showed typical apoptotic morphology when stained with acridine orange–ethidium bromide and hoechst staining. Cell cycle analysis clearly indicated that cell death was due to apoptosis. Enhancement in the proliferation of lymphocytes suggested immunomodulatory activity of this compound. To our best knowledge anticancer activity of 2-hydroxy-4-methoxy-3,6-dimethylbenzoic acid was reported here for thefirst time. Thus the results of the present study suggest that 2-hydroxy-4-methoxy-3,6-dimethylbenzoic acid has a strong potential to be developed as an antimicrobial and anticancer drug target after further clinical evaluation. 1. Introduction Multi-drug resistance is a world-wide problem, attributed to the extensive use of antibiotics, selection pressure on bacterial strains and lack of new drugs, vaccines and diagnostic aids. These shortcomings lead to an urgent global call for new antimicrobial drugs, particularly from natural resources.1 Meanwhile, the emergence of severe resistance to antibiotics in microbial pathogens, such as Gram-positive methicillin-resistant Staphylococcus aureus(MRSA) and vancomycin-res
URI: http://hdl.handle.net/123456789/2380
Appears in Collections:2014

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