Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/2587
Title: Preparation and Characterization of Selenium Incorporated Guar Gum Nanoparticle and Its Interaction with H9c2 Cells
Authors: Rema Sreenivasan, Soumya
Vadavanath Prabhakaran, Vineetha
Premachandran Latha, Reshma
Kozhiparambil Gopalan, Raghu
Keywords: Selenium Incorporated Guar Gum Nanoparticle
H9c2 Cells.
Issue Date: 30-Sep-2013
Publisher: PLOS ONE | www.plosone.org
Citation: Journal.pone.0074411; Volume 8 ;Issue 9 ;September 2013
Abstract: This study deals with the preparation and characterization of selenium incorporated guar gum nanoparticle (SGG), and its effect on H9c2 cardiomyoblast. Herein, nanoprecipitation techniques had been employed for the preparation of SGG nanoparticle. The prepared nanoparticle had been subjected to various types of analytical techniques like transmission electron microscopy (TEM), X-ray diffraction (XRD) and particle size analysis to confirm the characteristics of nanoparticle as well as for selenium incorporation. Physical characterization of nanoparticle showed that the size of nanoparticles increase upto ,69–173 nm upon selenium incorporation from ,41–132 nm. Then the prepared nanoparticles were evaluated for its effect on H9c2 cells. In this regard, the effect of nanoparticle on various vital parameters of H9c2 cells was studied. Parameters like cell viability, uptake of selenium incorporated guar gum nanoparticle by the cells, effect of SGG on DNA integrity, apoptosis, reactive oxygen species generation, alteration in transmembrane potential of mitochondria and cytoskeletal integrity had been investigated. Viability results showed that up to 25 nM of SGG was safe (10.31%) but beyond that it induces cytotoxicity. Cellular uptake of selenium showed that cell permeability for SGG is significantly high compared to normal selenium (7.2 nM of selenium for 25 nM SGG compared with 5.2 nM selenium for 25 nM sodium selenite). There was no apoptosis with SGG and also it protects DNA from hydroxyl radical induced breakage. Likewise no adverse effect on mitochondria and cytoskeleton was observed for 25 nM of SGG. Overall results reveal that SGG is highly suitable for biomedical research application.
URI: http://hdl.handle.net/123456789/2587
Appears in Collections:2013

Files in This Item:
File Description SizeFormat 
Preparation and Characterzatuion-RS Soumya.PDF
  Restricted Access
7.92 MBAdobe PDFView/Open Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.