Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/2896
Title: Immobilized Bacterial α-amylase for Effective Hydrolysis of Raw and Soluble Starch
Authors: Dhanya, G
Nampoothiri, K M
Swetha, S
Pandey, A
Keywords: Immobilization
Bacillus amyloliquefaciens
α-Amylase
Calcium alginate
Raw starch
Starch hydrolysis
Issue Date: May-2009
Publisher: Elsevier
Citation: Food Research International, 42(4):436-442
Abstract: The major concern in an enzymatic process is the instability of the enzyme under repetitive or prolonged use and inhibition by high substrate and product concentration. Immobilization is a very effective alternative in overcoming problems of instability and repetitive use of enzymes. Entrapment method of immobilization is advantageous over other methods as they do not involve chemical modification of the enzyme. α-Amylase produced by Bacillus amyloliquefaciens ATCC 23842 was immobilized in calcium alginate beads and used for the effective hydrolysis of soluble and raw potato starch which was comparable to the free enzyme. The levels of parameters (sodium alginate, calcium chloride and curing time) that significantly influence the immobilization of α-amylase in calcium alginate were analyzed and optimized using response surface methodology. Reactor studies were performed to study the reusability and operational stability of the beads. The alginate beads retained more than 60% of their initial efficiency after five batches of successive use and 40% of efficiency was exhibited in the 6th and 7th batch run of 6 h duration.
URI: http://hdl.handle.net/123456789/2896
Appears in Collections:2009

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