Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/3077
Full metadata record
DC FieldValueLanguage
dc.contributor.authorPreetha Rani, M R-
dc.contributor.authorAnupama, N-
dc.contributor.authorSreelekshmi, M-
dc.contributor.authorRaghu, K G-
dc.date.accessioned2018-06-01T09:07:48Z-
dc.date.available2018-06-01T09:07:48Z-
dc.date.issued2018-04-
dc.identifier.citationBiomedicine & Pharmacotherapy, 100:467-477en_US
dc.identifier.urihttp://10.10.100.66:8080/xmlui/handle/123456789/3077-
dc.description.abstractA series of cardiovascular complications associated with hyperglycemia is a critical threat to the diabetic population. Here we elucidate the link between hyperglycemia and cardiovascular diseases onset, focusing on oxidative stress and associated cardiac dysfunctions. The contribution of advanced glycation end products (AGE) and protein kinase C (PKC) signaling is extensively studied. For induction of hyperglycemia, H9c2 cells were incubated with 33 mM glucose for 48 h to simulate the diabetic condition in in vitro system. Development of cardiac dysfunction was confirmed with the significant increase of lactate dehydrogenase (LDH) release to the medium and associated decrease in cell viability. Various parameters like free radical generation, alteration in innate antioxidant system, lipid peroxidation, AGE production and PKC α -ERK axis were investigated during hyperglycemia and with chlorogenic acid. Hyperglycemia has significantly enhanced reactive oxygen species (ROS- 4 fold) generation, depleted SOD activity (1.3 fold) and expression of enzymes particularly CuZnSOD (SOD1) and MnSOD (SOD2), increased production of AGE (2.18 fold). Besides, PKC α dependent ERK signaling pathway was found activated (1.43 fold) leading to cardiac dysfunction during hyperglycemia. Chlorogenic acid (CA) was found beneficial against hyperglycemia most probably through its antioxidant mediated activity. The outcome of this preliminary study reveals the importance of integrated approach emphasizing redox status, glycation and signaling pathways like PKC α - ERK axis for control and management of diabetic cardiomyopathy (DCM) and potential of bioactives like CA.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectHyperglycemiaen_US
dc.subjectH9c2 cellsen_US
dc.subjectChlorogenic aciden_US
dc.subjectAdvanced glycated end productsen_US
dc.subjectProtein kinase Cen_US
dc.titleChlorogenic Acid Attenuates Glucotoxicity in H9c2 Cells Via Inhibition of Glycation and PKC α Upregulation and Safeguarding Innate Antioxidant Statusen_US
dc.typeArticleen_US
Appears in Collections:2018

Files in This Item:
File Description SizeFormat 
Chlorogenic acid attenuates glucotoxicity - Preetha Rani M R - Biomedicine & Pharmacotherapy.pdf
  Restricted Access
1.84 MBAdobe PDFView/Open Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.