Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/3168
Title: Real Time Imaging and Dynamics of Hippocampal Zn2+ Under Epileptic Condition Using a Ratiometric Fluorescent Probe
Authors: Santhakumar, H
Nair, R V
Divya, S P
Shenoy, S J
Anoopkumar, T
Ajayaghosh, A
Jayasree, R S
Issue Date: 1-Jul-2018
Publisher: Springer
Citation: Scientific Reports, 8(1):9069
Abstract: Zinc, the essential trace element in human body exists either in the bound or free state, for both structural and functional roles. Insights on Zn2+ distribution and its dynamics are essential in view of the fact that Zn2+ dyshomeostasis is a risk factor for epileptic seizures, Alzheimer’s disease, depression, etc. Herein, a bipyridine bridged bispyrrole (BP) probe is used for ratiometric imaging and quantification of Zn2+ in hippocampal slices. The green fluorescence emission of BP shifts towards red in the presence of Zn2+. The probe is used to detect and quantify the exogenous and endogenous Zn2+ in glioma cells and hippocampal slices. The dynamics of chelatable zinc ions during epileptic condition is studied in the hippocampal neurons, in vitro wherein the translocation of Zn2+ from presynaptic to postsynaptic neuronal bodies is imaged and ratiometrically quantified. Raman mapping technique is used to confirm the dynamics of Zn2+ under epileptic condition. Finally, the Zn2+ distribution was imaged in vivo in epileptic rats and the total Zn2+ in rat brain was quantified. The results favour the use of BP as an excellent Zn2+ imaging probe in biological system to understand the zinc associated diseases and their management.
Appears in Collections:2018

Files in This Item:
File Description SizeFormat 
Real Time Imaging and Dynamics of Hippocampa-Santhakumar-Scientific Reports.pdf
  Restricted Access
3.91 MBAdobe PDFView/Open Request a copy

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.