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DC Field | Value | Language |
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dc.contributor.author | Santhakumar, H | - |
dc.contributor.author | Nair, R V | - |
dc.contributor.author | Divya, S P | - |
dc.contributor.author | Shenoy, S J | - |
dc.contributor.author | Anoopkumar, T | - |
dc.contributor.author | Ajayaghosh, A | - |
dc.contributor.author | Jayasree, R S | - |
dc.date.accessioned | 2018-07-06T11:02:14Z | - |
dc.date.available | 2018-07-06T11:02:14Z | - |
dc.date.issued | 2018-07-01 | - |
dc.identifier.citation | Scientific Reports, 8(1):9069 | en_US |
dc.identifier.uri | http://10.10.100.66:8080/xmlui/handle/123456789/3168 | - |
dc.description.abstract | Zinc, the essential trace element in human body exists either in the bound or free state, for both structural and functional roles. Insights on Zn2+ distribution and its dynamics are essential in view of the fact that Zn2+ dyshomeostasis is a risk factor for epileptic seizures, Alzheimer’s disease, depression, etc. Herein, a bipyridine bridged bispyrrole (BP) probe is used for ratiometric imaging and quantification of Zn2+ in hippocampal slices. The green fluorescence emission of BP shifts towards red in the presence of Zn2+. The probe is used to detect and quantify the exogenous and endogenous Zn2+ in glioma cells and hippocampal slices. The dynamics of chelatable zinc ions during epileptic condition is studied in the hippocampal neurons, in vitro wherein the translocation of Zn2+ from presynaptic to postsynaptic neuronal bodies is imaged and ratiometrically quantified. Raman mapping technique is used to confirm the dynamics of Zn2+ under epileptic condition. Finally, the Zn2+ distribution was imaged in vivo in epileptic rats and the total Zn2+ in rat brain was quantified. The results favour the use of BP as an excellent Zn2+ imaging probe in biological system to understand the zinc associated diseases and their management. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Springer | en_US |
dc.title | Real Time Imaging and Dynamics of Hippocampal Zn2+ Under Epileptic Condition Using a Ratiometric Fluorescent Probe | en_US |
dc.type | Article | en_US |
Appears in Collections: | 2018 |
Files in This Item:
File | Description | Size | Format | |
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Real Time Imaging and Dynamics of Hippocampa-Santhakumar-Scientific Reports.pdf Restricted Access | 3.91 MB | Adobe PDF | View/Open Request a copy |
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