Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/371
Title: Characterization of laccase isoforms produced by pleurotus ostreatus in solid state fermentation of sugarcane bagasse
Authors: Karp, S G
Faraco, V
Amore, A
Birolo, L
Giangrande, C
Soccol, V T
Pandey, A
Soccol, C R
Keywords: Laccase isoforms
Sugarcane bagasse
Solid state fermentation
Pleurotus ostreatus
Zymogram
Issue Date: Jun-2012
Publisher: Elsevier
Citation: Bioresource Technology 114:735-739;2012
Abstract: Laccases are oxidative enzymes linked to biological degradation of lignin.The aim of this work was to evaluate the effect of inducers and different concentrations of nitrogen on production level of total laccase activity and pattern of laccase isoforms, produced in solid state fermentation of sugarcane bagasse by a selected strain of Pleurotus ostreatus. The addition of yeast extract 5 g/L, copper sulfate 150 lM and ferulic acid 2 mM provided highest enzymatic activity (167 U/g) and zymograms indicated the presence of six laccase isoforms(POXA1b, POXA3, POXC and three other isoforms). Results of protein identification by mass spectrometry confirmed the presence of POXC and POXA3 as the main isoenzymes, and also identified a glyoxal oxidase and three galactose oxidases. The fact that the isoenzyme POXA1b was not identified in the analyzed samples can be possibly explained by its sensitivity to protease degradation.
URI: http://hdl.handle.net/123456789/371
Appears in Collections:2012

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