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Title: High Yield recovery of 2,3-Butanediol from Fermented Broth Accumulated on Xylose Rich Sugarcane Bagasse Hydrolysate Using Aqueous Two-Phase Extraction System
Authors: Narisetty, V
Amraoui, Y
Abdullah, A
Ahmad, E
Agrawal, D
Parameswaran, B
Pandey, A
Goel, S
Kumar, V
Keywords: xylose,
enterobacter ludwigii,
aqueous two-phase system,
ammonium sulphate,
Issue Date: Oct-2021
Publisher: Elsevier
Citation: Bioresource Technology; 337: 125463
Abstract: Downstream processing of chemicals obtained from fermentative route is challenging and cost-determining factor of any bioprocess. 2,3-Butanediol (BDO) is a promising chemical building block with myriad applications in the polymer, food, pharmaceuticals, and fuel sector. The current study focuses on the recovery and purification of BDO produced (68.2 g/L) from detoxified xylose-rich sugarcane bagasse hydrolysate by a mutant strain of Enterobacter ludwigii. Studies involving screening and optimization of aqueous-two phase system (ATPS) revealed that 30% w/v (NH4)2SO4 addition to clarified fermented broth facilitated BDO extraction in isopropanol (0.5 v/v), with maximum recovery and partition coefficient being 97.9 ± 4.6% and 45.5 ± 3.5, respectively. The optimized protocol was repeated with unfiltered broth containing 68.2 g/L BDO, cell biomass, and unspent protein, which led to the partitioning of 66.7 g/L BDO, 2.0 g/L xylose and 9.0 g/L acetic acid into organic phase with similar BDO recovery (97%) and partition coefficient (45).
Appears in Collections:2021

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