Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/4441
Title: Monitoring Glutathione Dynamics in DNA Replication (S-phase) Using a Two-photon Reversible Ratiometric Fluorescent Probe
Authors: Biswas, S
Sarkar, S
Dhamija, A
Murali, V P
Maiti, K K
Bhuniya, S,
Keywords: glutathione
DNA replication
Issue Date: 2023
Publisher: Royal society of chemistry
Citation: Journal of Materials Chemistry B;11(9):1948-1957
Abstract: The redox regulator glutathione (GSH) migrates to the nucleus to give a safeguard to DNA replication in the S-phase. The fluctuation of GSH dynamics in the cell cycle process may help to understand cancerogenesis or other abnormalities in DNA replication. For the first time, we attempted to track the time-dependent S-phase change using the newly developed ratiometric fluorescent probe Nu-GSH. This probe is highly chemoselective towards glutathione and shows an emission intensity shift from 515 nm to 455 nm. It has shown fluorescence reversibility from blue to green channels while scavenging reactive oxygen species H2O2. Both ratiometric fluorescence images and FACS analysis have provided quantitative information on the GSH levels in the nucleoli during DNA replication in the S-phase. Furthermore, GSH fluctuation reciprocated the decay of the S-phase on a time scale. Additionally, its two-photon ability guaranteed its capability to study GSH dynamics in live cells/tissues noninvasively. We envision that the probe Nu-GSH can be used to get high-throughput quantitative information on glutathione dynamics and give an opportunity to monitor its perturbation during the course of cell division.
Appears in Collections:2023

Files in This Item:
File Description SizeFormat 
Monitoring glutathione dynamics in DNA_Biswas_Journal of materials chemistry B.pdf
  Restricted Access
3.04 MBAdobe PDFView/Open Request a copy

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.