Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/4599
Full metadata record
DC FieldValueLanguage
dc.contributor.authorNair, K S-
dc.contributor.authorRadhakrishnan, S-
dc.contributor.authorBajaj, H-
dc.date.accessioned2023-11-06T13:54:20Z-
dc.date.available2023-11-06T13:54:20Z-
dc.date.issued2023-07-21-
dc.identifier.citationACS Synthetic Biology;12(7):2168-2177en_US
dc.identifier.urihttps://doi.org/10.1021/acssynbio.3c00249-
dc.identifier.urihttp://localhost:8080/xmlui/handle/123456789/4599-
dc.description.abstractMembrane-less compartments formed via liquid− liquid phase separation (LLPS) are regulated dynamically via enzyme reactions in cells. Giant unilamellar vesicles (GUVs) provide a promising chassis to control, mimic, and understand the LLPS process; however, they are challenging to construct. Here, we engineer the dynamic assembly and disassembly of LLPS compartments using complex coacervates as models inside synthetic cells. Semipermeable GUVs constructed with defined lipid composition encapsulate the biomolecules, including enzymes required to regulate coacervates. Assembly and disassembly of coacervates are triggered in independent systems by the diffusion of substrates through the membrane into the vesicle lumen. The coupling of enzyme networks in a single synthetic cell system allows for reversible and out-of-equilibrium regulation of coacervates. The functional properties of the coacervates are revealed by sequestering biomolecules, including drugs and enzymes. GUVs, with functional LLPS compartment assembly, open avenues in constructing programmable autonomous synthetic cells with membraneless organelles. The coacervate-in-vesicle platform has significant implications for understanding LLPS regulation mechanisms in cells.en_US
dc.language.isoenen_US
dc.publisherACS Publicationsen_US
dc.subjectgiant unilamellar vesicles,en_US
dc.subjectcompartmentalizationen_US
dc.subjectliquid−liquid phase separationen_US
dc.subjectcomplex coacervatesen_US
dc.subjectout-of-equilibrium systemen_US
dc.subjectsynthetic cellsen_US
dc.titleDynamic Control of Functional Coacervates in Synthetic Cellsen_US
dc.typeArticleen_US
Appears in Collections:2023

Files in This Item:
File Description SizeFormat 
Dynamic Control of Functional Coacervates in Synthetic Cells_KarthikaSNair_ACS Synthetic Biology.pdf
  Restricted Access
6.23 MBAdobe PDFView/Open Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.