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Title: | Statistical optimization of simultaneous saccharification and L(+)-lactic acid fermentation from cassava bagasse using mixed culture of lactobacilli by response surface methodology |
Authors: | John, R P Rajeev K Sukumaran Nampoothiri, K M Pandey, A |
Keywords: | Cassava bagasse L(+)- Lactic acid Response surface optimization Saccharification Fermentation Lactobacillus |
Issue Date: | 1-Oct-2007 |
Publisher: | Elsevier |
Citation: | Biochemical Engineering Journal 36(3):262-267;1 Oct 2007 |
Abstract: | Optimization of five process parameters (concentrations of cassava bagasse, enzyme, yeast extract, NH4Cl and inoculum) was attempted using a Box-Behnken design for the optimal production of L(+)-lactic acid by a mixed culture of Lactobacillus casei and Lactobacillus delbrueckii by simultaneous saccharification and fermentation. Maximum lactic acid yield of 81 g/L was obtained when 15% (w/v)cassava bagasse treated with 12.5 mL/L enzyme mixture was supplemented with 7.5 g/L yeast extract and 3 g/L NH4Cl and inoculated with 3 x 10 rise to 10 CFU/L of lactobacilli and incubated for 60 h at 37°C as static culture. |
URI: | http://hdl.handle.net/123456789/51 |
Appears in Collections: | 2007 |
Files in This Item:
File | Description | Size | Format | |
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2007_00139.pdf Restricted Access | 178.69 kB | Adobe PDF | View/Open Request a copy |
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