HPTLC estimation of oroxylin A in oroxylum indicum herbal formulations

Abstract

Oroxylum indicum (L.) Kurz is an important medicinal plant with a wide spectrum of medicinal properties. It is frequently used in a large number of traditional medicinal formulations. Oroxylin A is considered as one of the major bioactive compounds in O. indicum. Optimization of semi-preparative high-performance liquid chromatography (HPLC) conditions for the isolation of oroxylin A from the plant parts and development of a high-performance thin-layer liquid chromatography (HPTLC) analytical method for the quantification of oroxylin A in polyherbal formulations are reported in this study. The HPTLC method was validated in terms of its linearity, LOQ, precision, and accuracy and was demonstrated for the quantification of oroxylin A in O. indicum plant parts and some commercial “Ayurvedic polyherbal formulations”. The method was able to identify and quantify oroxylin Ain complex mixtures of phytochemicals and could be extended to the marker-based standardization of other polyherbal formulations.