Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/662
Title: Co-culturing of lactobacillus paracasei subsp paracasei with a lactobacillus delbrueckii subsp delbrueckii mutant to make high cell density for increased lactate productivity from cassava bagasse hydrolysate
Authors: John, R P
Nampoothiri, K M
Keywords: Solid state fermentation
Lactic acid production
Simultaneous saccharification
Issue Date: 2011
Publisher: Springer
Citation: Current Microbiology 62(3):790-794;Mar 2011
Abstract: To increase the productivity of lactic acid, a co-culture of lactobacilli was made by mixing 1:1 ratio of Lactobacillus paracasei subsp. paracasei and a fast growing L. delbrueckii subsp. delbrueckii mutant. The culture was embedded on to polyurethane foam (PUF) cubes as a biofilm and used for fermentation. In order to prevent the cell leakage, the PUF cubes were further entrapped in calcium cross-linked alginate. The maximum lactic acid production using a high cell density free culture was > 38 g l(-1) from similar to 40 g l(-1) of reducing sugar within 12 h of fermentation. Using PUF biofilms, the same yield of lactic acid attained after 24 h. When the cubes were further coated with alginate it took 36 h for the maximum yield. Even though, the productivity is slightly lesser with the alginate coating, cell leakage was decreased and cubes were reused without much decrease in production in repeated batches. Using a conventional control inoculum (3%, w/v), it took 120 h to yield same amount of lactic acid
Appears in Collections:2011

Files in This Item:
File Description SizeFormat 
2011_ 0003.pdf
  Restricted Access
232.62 kBAdobe PDFView/Open Request a copy

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.