Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/719
Title: Media engineering for production of poly-beta-hydroxybutyrate by Bacillus firmus NII 0830
Authors: Deepthi, S K
Binod, P
Sindhu, R
Pandey, A
Keywords: Biodiesel industry
Poly-beta-hydroxybutyrate (PHB)
Response surface methodology (RSM)
Recombinant escherichia-coli
Polyhydroxybutyrate production
Issue Date: 2011
Publisher: NISCAIR
Citation: Journal of Scientific & Industrial Research 70(11):968-975;Nov 2011
Abstract: This study evaluates biodiesel industry generated crude glycerol as sole carbon source for the production of poly-beta-hydroxybutyrate (PHB) using Bacillus firm us NII 0830. Pretreatment of crude glycerol from biodiesel industry was tried using strong acids. Biomass yield and PHB accumulation by B. firm us Nil 0830 in submerged fermentation was compared using crude as well as pretreated glycerol as sole carbon source. It was observed that crude glycerol from biodiesel industry, without any pretreatment, can be used for PHB production. The organism when grown in mineral salt media containing crude glycerol as carbon source gave a maximum yield of biomass (3 g/l) and PHB (53% of cell dry wt). Response surface methodology (RSM) showed that presence of nitrogen and phosphate in fermentation media favored biomass production but reduced PHB production. Optimized crude glycerol media for maximum PHB accumulation contains ammonium sulphate (4 g/l) and K(2)HPO(4) (0.8 g/l).
ISSN: 0022-4456
Appears in Collections:2011

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