Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/825
Title: Polyphasic taxonomy of novel actinobacteria showing macromolecule degradation potentials in Bigeum Island, Korea
Authors: Dastager, S G
Pandey, A
Lee, J-C
Li, W-J
Kim, C-J
Keywords: Microbial diversity
Polyphasic approach
Evolution
Chromatography
Mesophilum SP- NOV
Bigeum Island
Issue Date: 2009
Publisher: Springer
Citation: Current Microbiology 59(1):21-29;Jul 2009
Abstract: Aerobic, alkaliphilic to alkalitolerant and mesophilic bacteria were isolated and characterized from soil and sediment samples collected from Bigeum Island, South Korea. The total numbers of microorganisms in the soil and sediment samples were found to be 10(3)-10(5) cfu/g and 10(2)-10(7) cfu/g, respectively. A total of 163 isolates were isolated and subjected to further characterization on the basis of pH, temperature and salt tolerance. Among the 163 isolates, 54 were selected based on their tolerance attributes to temperature, pH and NaCl. Out of the 54 isolates, 27 were further selected based on their multiple tolerance ability and enzyme profile and were subjected to 16S rRNA gene sequencing and phylogenetic analysis. The latter indicated that most of the Bigeum Island isolates were related to the phylum Actinobacteria. The phylogenetic tree based on 16S rRNA gene sequences placed the 27 isolates into 9 different major bacterial genera, each genus comprising pure cultures that shared a parts per thousand currency sign97% sequence identity and 18 putative novel species. Most of the strains were alkalitolerant and mesophilic, and produced biotechnologically important enzymes at alkaline pH.
URI: http://ir.niist.res.in:8080/jspui/handle/123456789/825
ISSN: 0343-8651
Appears in Collections:2009

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