1. DSpace at My University
  2. Journal articles
  3. 2009
Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/841
Title: Production and partial purification of alpha-amylase from a novel isolate streptomyces gulbargensis
Authors: Dastager, S G
Agasar, D
Pandey, A
Keywords: Streptomyces
Amylolytic enzymes
Purification
pH and temperature effect
Enzymatic properties
Alkaliphilic bacillus isolate
Extracellular amylase
Issue Date: 2009
Publisher: Springer
Citation: Journal of Industrial Microbiology & Biotechnology 36(2):189-194;Feb 2009
Abstract: Extracellular amylase production by a newly isolated alkali-thermotolerant strain Streptomyces gulbargensis DAS 131 was optimized and characterized. The highest amylase production was achieved by growing S. gulbargensis DAS 131 in media with 1% starch. Strain exhibited maximal activity at pH 9.0 and 45A degrees C and relatively stable in alkaline conditions (pH 11). Starch and peptone were found to be the good source of carbon and nitrogen with a yield of 2,216.6 and 2,156.1 U, respectively. Maltose and maltotriose were the main end products of starch hydrolysis, indicating alpha-amylase activity. SDS-PAGE analysis revealed a monomeric form with a molecular weight of 55 kDa.
URI: http://ir.niist.res.in:8080/jspui/handle/123456789/841
ISSN: 1367-5435
Appears in Collections:2009

Files in This Item:
File Description SizeFormat 
2009 _ 00079.pdf
  Restricted Access		441.92 kBAdobe PDFView/Open Request a copy
Show full item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.