Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/842
Title: Cytotoxicity and fluorescence studies of silica-coated CdSe quantum dots for bioimaging applications
Authors: Vibin, M
Vinayakan, R
Annie John
Raji, V
Rejiya, C S
Vinesh, N S
Annie Abraham
Keywords: Cytotoxicity
Quantum dots
Semiconductors
Luminescence
Health safety
Silica-coating
EHS
Surface modification
Nanoparticles
Toxicity
Semiconductor
Issue Date: 2011
Publisher: Springer
Citation: Journal of Nanoparticle Research 13(6):2587-2596;Jun 2011
Abstract: The toxicological effects of silica-coated CdSe quantum dots (QDs) were investigated systematically on human cervical cancer cell line. Trioctylphosphine oxide capped CdSe QDs were synthesized and rendered water soluble by overcoating with silica, using aminopropyl silane as silica precursor. The cytotoxicity studies were conducted by exposing cells to freshly synthesized QDs as a function of time (0-72 h) and concentration up to micromolar level by Lactate dehydrogenase assay, MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay, Neutral red cell viability assay, Trypan blue dye exclusion method and morphological examination of cells using phase contrast microscope. The in vitro analysis results showed that the silica-coated CdSe QDs were nontoxic even at higher loadings. Subsequently the in vivo fluorescence was also demonstrated by intravenous administration of the QDs in Swiss albino mice. The fluorescence images in the cryosections of tissues depicted strong luminescence property of silica-coated QDs under biological conditions. These results confirmed the role of these luminescent materials in biological labeling and imaging applications.
URI: http://ir.niist.res.in:8080/jspui/handle/123456789/842
ISSN: 1388-0764
Appears in Collections:2011

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