Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/914
Title: Amino acid production from rice straw and wheat bran hydrolysates by recombinant pentose-utilizing Corynebacterium glutamicum
Authors: Vipin Gopinath
Meiswinkel, T M
Wendisch, V F
Nampoothiri, K M
Keywords: Corynebacterium glutamicum
Amino acid production
Lignocellulosic hydrolysates
Pentose utilization
Renewables
Metabolic engineering
Ethanologenic escherichia-coli
Complete genome sequence
Saccharomyces-cerevisiae
Lysine production
L-arabinose
Carbon-sources
Heterologous expression
Glutamate production
Metabolic pathway
Organic-acids
Issue Date: 2011
Publisher: Springer
Citation: Applied Microbiology and Biotechnology 92(5):985-996; Dec 2011
Abstract: Corynebacterium glutamicum wild type lacks the ability to utilize the pentose fractions of lignocellulosic hydrolysates, but it is known that recombinants expressing the araBAD operon and/or the xylA gene from Escherichia coli are able to grow with the pentoses xylose and arabinose as sole carbon sources. Recombinant pentose-utilizing strains derived from C. glutamicum wild type or from the l-lysine-producing C. glutamicum strain DM1729 utilized arabinose and/or xylose when these were added as pure chemicals to glucose-based minimal medium or when they were present in acid hydrolysates of rice straw or wheat bran. The recombinants grew to higher biomass concentrations and produced more l-glutamate and l-lysine, respectively, than the empty vector control strains, which utilized the glucose fraction. Typically, arabinose and xylose were co-utilized by the recombinant strains along with glucose either when acid rice straw and wheat bran hydrolysates were used or when blends of pure arabinose, xylose, and glucose were used. With acid hydrolysates growth, amino acid production and sugar consumption were delayed and slower as compared to media with blends of pure arabinose, xylose, and glucose. The ethambutol-triggered production of up to 93 +/- 4 mM l-glutamate by the wild type-derived pentose-utilizing recombinant and the production of up to 42 +/- 2 mM l-lysine by the recombinant pentose-utilizing lysine producer on media containing acid rice straw or wheat bran hydrolysate as carbon and energy source revealed that acid hydrolysates of agricultural waste materials may provide an alternative feedstock for large-scale amino acid production.
URI: http://ir.niist.res.in:8080/jspui/handle/123456789/914
ISSN: 0175-7598
Appears in Collections:2011

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