Browsing by Author "Prema, P"

Effect of inducers and process parameters on laccase production by Streptomyces psammoticus and its application in dye decolourization

Niladevi, K N; Prema, P (Elsevier, 2008)

The process parameters influencing the production of extracellular laccases by Streptomyces psammoticus MTCC 7334 were optimized in submerged fermentation. Coffee pulp and yeast extract were the best substrate and nitrogen ...

Evaluation of alpha-galactosidase biosynthesis by Streptomyces griseoloalbus in solid-state fermentation using response surface methodology

Anisha, G S; Rajeev K Sukumaran; Prema, P (Wiley, 2008)

Aim: The present study was aimed at evaluating the effects of the three crucial factors, galactose concentration, inoculum size and moisture content, on alpha-galactosidase production by the filamentous actinobacterium ...

Expression of rice (Oryza sativa L. var. Nipponbare) alpha-galactosidase genes in Escherichia coli and characterization

Li, S; Kim, Wook-Dong; Kaneko, S; Prema, P; Nakajima, M; Kobayashi, H (Taylor & Francis, 2007)

Two putative et-galactosidase genes from rice (Oryza sativa L. var. Nipponbare) belonging to glycoside hydrolase family 27 were cloned and expressed in Escherichia coli. These enzymes showed alpha-galactosidase activity ...

Hydrolysis of pectin: An enzymatic approach and its application in banana fiber processing

Nicemol Jacob; Niladevi, K N; Anisha, G S; Prema, P (Elsevier, 2008)

Sediment samples were collected from different estuarine and marine areas along the West coast of India. Eighteen actinomycete cultures were isolated using starch casein agar and were screened for polygalacturonase activity ...

Immobilization of laccase from Streptomyces psammoticus and its application in phenol removal using packed bed reactor

Niladevi, K N; Prema, P (Springer, 2008)

Laccase was produced from Streptomyces psammoticus under solid-state fermentation. The enzyme was partially purified by ammonium sulphate precipitation and was immobilized in alginate beads by entrapment method. Calcium ...

Influence of mode of fermentation on production of polygalacturonase by a novel strain of Streptomyces lydicus

Nicemol Jacob; Prema, P (Faculty Food technology and Biotechnology, 2006)

Production of different pectinolytic enzymes was attempted using the actinomycete strain Streptomyces lydicus in submerged fermentation. Polygalacturonase and pectin lyase activities were detected in the culture supernatant, ...

Investigation on alpha-galactosidase production by streptomyces griseoloalbus in a forcefully aerated packed-bed bioreactor operating in solid-state fermentation condition

Anisha, G S; Rojan P John; Prema, P; Pandey, A (Springer, 2010)

Solid-state fermentation (SSF) was carried out for the production of extracellular alpha-galactosidase by Streptomyces griseoloalbus. Soybean flour was the best solid substrate for alpha-galactosidase production. Packed-bed ...

Novel process for the simultaneous extraction and degumming of banana fibers under solid-state cultivation

Nicemol Jacob; Prema, P (Sociedade Brasileira de Microbiologia, 2008-01)

Various process parameters for the production of polygalacturonase by Streptomyces lydicus under solid-state fermentation were optimized. The optimum particle size of wheat bran for polygalacturonase production was in the ...

Optimization of enzymatic clarification of sapodilla juice: A statistical perspective

Nicemol Jacob; Rajeev K Sukumaran; Prema, P (Springer, 2008)

Response surface methodology (RSM) was employed to establish optimum conditions for enzymatic clarification of sapodilla juice. Polygalacturonase obtained from Streptomyces lydicus had been purified to homogeneity and was ...

Optimization of laccase production from a novel strain-Streptomyces psammoticus using response surface methodology

Niladevi, K N; Rajeev K Sukumaran; Nicemol Jacob; Anisha, G S; Prema, P (Elsevier, 2009)

Response surface methodology was employed for the optimization of different nutritional and physical parameters for the production of laccase by the filamentous bacteria Streptomyces psammoticus MTCC 7334 in submerged ...

Production and characterization of partially purified thermostable alpha-galactosidases from Streptomyces griseoloalbus for food industrial applications

Anisha, G S; Rojan P John; Nicemol, J; Niladevi, K N; Prema, P (Elsevier, 2008)

Solid-state fermentation was carried out for the production of extracellular alpha-galactosidase by Streptomyces griseoloalbus. Soybean flour was the best solid substrate for alpha-galactosidase production. In flask-level ...

Production and partial characterization of endoxylanase by Bacillus pumilus using agro industrial residues

Asha Poorna, C; Prema, P (Elsevier, 2006)

Agro industrial residues, cheap sources of energy have high potential in the area of fermentation for the production of lignocellulases. Different agro industrial waste products were evaluated as substrates in submerged ...

Production of alpha-galactosidase by a novel actinomycete streptomyces griseoloalbus and its application in soymilk hydrolysis

Anisha, G S; Prema, P (Springer, 2007)

alpha-Galactosidase production by a newly isolated actinomycete Streptomyces griseoloalbus under submerged fermentation was investigated. The influence of initial pH of medium, incubation temperature, inoculum age and ...

Production of cellulase-free endoxylanase from novel alkalophilic thermotolerent Bacillus pumilus by solid-state fermentation and its application in wastepaper recycling

Asha Poorna, C; Prema, P (Elsevier, 2007)

The present study aimed at optimization of culture condition for the enhanced production of extra cellular thermostable cellulase-free xylanase from Bacillus pumilus by solid-state fermentation. Batch studies were carried ...

Purification and partial characterization of polygalacturonase from Streptomyces lydicus

Nicemol Jacob; Asha Poorna, C; Prema, P (Elsevier, 2008)

Polygalacturonase produced by Streptomyces lydicus was purified to homogeneity by ultrafiltration and a combination of ion exchange and gel filtration chromatographic procedures. The purified enzyme was an exo-polygalacturonase ...

Reduction of non-digestible oligosaccharides in horse gram and green gram flours using crude alpha-galactosidase from Streptomyces griseoloalbus

Anisha, G S; Prema, P (Elsevier, 2008)

The effectiveness of using crude extracellular alpha-galactosidase from Streptomyces griseoloalbus for the treatment of horse gram and green gram flours was investigated by comparing with traditional treatments such as ...

Selection of optimal growth medium for the synthesis of α-galactosidase from mangrove actinomycetes

Anisha, G S; Prema, P (Niscair, 2006)

-Galactosidases ( -galactoside galactohydrolase, EC.3.2.1.22) are exoglycosidases that cleave terminal -1→6-linked galactose residues from glycoconjugates. To select best medium for the enhanced production of this enzyme ...

Solid-state fermentation of lignocellulosic substrates for cellulase production by Trichoderma reesei NRRL 11460

Singhania, R R; Rajeev K Sukumaran; Anu Pillai; Prema, P; Szakacs, G; Pandey, A (CSIR, 2006)

Cellulase production studies were carried out using the fungal culture Trichoderma reesei NRRL 11460 using four different lignocellulosic residues (both raw and pre-treated) by solid-state fermentation. The effect of basic ...

Statistical optimization of alpha-galactosidase production in submerged fermentation by Streptomyces griseoloalbus using response surface methodology

Anisha, G S; Rajeev K Sukumaran; Prema, P (Faculty Food Technology Biotechnology, 2008)

alpha-Galactosidase production by a novel actinomycete strain Streptomyces griseoloalbus in shake flask culture was optimized using response surface methodology. Screening of variables to find their relative effect on ...

Strategies for enhancing laccase yield from Streptomyces psammoticus and its role in mediator-based decolorization of azo dyes

Niladevi, K N; Sheejadevi, P S; Prema, P (Springer, 2008)

Enhanced production of laccases from Streptomyces psammoticus in solid-state fermentation was carried out using two different strategies: laccase inducers and scale-up process. Laccase yield was enhanced by a wide range ...

Browsing by Author "Prema, P"

Browsing by Author "Prema, P"

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