Investigation on alpha-galactosidase production by streptomyces griseoloalbus in a forcefully aerated packed-bed bioreactor operating in solid-state fermentation condition

Abstract

Solid-state fermentation (SSF) was carried out for the production of extracellular alpha-galactosidase by Streptomyces griseoloalbus. Soybean flour was the best solid substrate for alpha-galactosidase production. Packed-bed bioreactor performed well in enhancing the enzyme yield and resulted in a highest yield of 197.2 +/- 0.02 U/gds with a forced aeration of 2 vvm, which was approximately twofold the yield obtained in flasks. The alpha-galactosidase production was growth-associated, and the highest enzyme yield was obtained after 96 h of incubation. It is significant that this is the first report on alpha-galactosidase production by a filamentous bacterium in SSF using packed-bed bioreactor.