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Compactin production studies using penicillium brevicompactum under solid-state fermentation conditions

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dc.contributor.author Shaligram, N S
dc.contributor.author Singh, S K
dc.contributor.author Singhal, R S
dc.contributor.author Pandey, A
dc.contributor.author Szakacs, G
dc.date.accessioned 2014-01-21T09:52:15Z
dc.date.available 2014-01-21T09:52:15Z
dc.date.issued 2009
dc.identifier.citation Applied Biochemistry and Biotechnology 159(2):505-520;Nov 2009 en_US
dc.identifier.issn 0273-2289
dc.identifier.uri http://ir.niist.res.in:8080/jspui/handle/123456789/1089
dc.description.abstract In the present study, compactin production by Penicillium brevicompactum WA 2315 was optimized using solid-state fermentation. The initial one factor at a time approach resulted in improved compactin production of 905 mu g gds(-1) compared to initial 450 mu g gds(-1). Subsequently, nutritional, physiological, and biological parameters were screened using fractional factorial and Box-Behnken design. The fractional factorial design studied inoculum age, inoculum volume, pH, NaCl, NH(4)NO(3), MgSO(4), and KH(2)PO(4). All parameters were found to be significant except pH and KH(2)PO(4). The Box-Behnken design studied inoculum volume, inoculum age, glycerol, and NH(4)NO(3) at three different levels. Inoculum volume (p=0.0013) and glycerol (p=0.0001) were significant factors with greater effect on response. The interaction effects were not significant. The validation study using model-defined conditions resulted in an improved yield of 1,250 mu g gds(-1) compactin. Further improvement in yield was obtained using fed batch mode of carbon supplementation. The feeding of glycerol (20% v/v) on day 3 resulted in further improved compactin yield of 1,406 mu g gds(-1). The present study demonstrates that agro-industrial residues can be successfully used for compactin production, and statistical experiment designs provide an easy tool to improve the process conditions for secondary metabolite production. en_US
dc.language.iso en en_US
dc.publisher Humana Press en_US
dc.subject Box–Behnken design en_US
dc.subject Streptomyces en_US
dc.subject Glycerol en_US
dc.subject Clavulanic acid production en_US
dc.subject Filamentous fungi en_US
dc.subject Compactin en_US
dc.subject Penicillium brevicompactum WA 2315 en_US
dc.subject Solid-state fermentation en_US
dc.subject Fractional factorial design en_US
dc.title Compactin production studies using penicillium brevicompactum under solid-state fermentation conditions en_US
dc.type Article en_US

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