DSpace Repository

Lipase from marine Aspergillus awamori BTMFW032: Production, partial purification and application in oil effluent treatment

Show simple item record

dc.contributor.author Basheer, S M
dc.contributor.author Sreeja, C
dc.contributor.author Beena, P S
dc.contributor.author Rajeev K Sukumaran
dc.contributor.author Elyas, K K
dc.contributor.author Chandrasekaran, M
dc.date.accessioned 2014-01-22T06:09:33Z
dc.date.available 2014-01-22T06:09:33Z
dc.date.issued 2011
dc.identifier.citation New Biotechnology 28(6):627-638;Oct 2011 en_US
dc.identifier.issn 1871-6784
dc.identifier.uri http://ir.niist.res.in:8080/jspui/handle/123456789/1100
dc.description.abstract Marine fungus BTMFW032, isolated from seawater and identified as Aspergillus awamori, was observed to produce an extracellular lipase, which could reduce 92% fat and oil content in the effluent laden with oil. In this study, medium for lipase production under submerged fermentation was optimized statistically employing response surface method toward maximal enzyme production. Medium with soyabean meal-0.77% (w/v); (NH(4))(2)SO(4)-0.1 M; KH(2)PO(4)-0.05 M; rice bran oil-2% (v/v); CaCl(2)-0.05 M; PEG 6000-0.05% (w/v); NaCl-1% (w/v); inoculum-1% (v/v); pH 3.0; incubation temperature 35 degrees C and incubation period-five days were identified as optimal conditions for maximal lipase production. The time course experiment under optimized condition, after statistical modeling, indicated that enzyme production commenced after 36 hours of incubation and reached a maximum after 96 hours (495.0 U/ml), whereas maximal specific activity of enzyme was recorded at 108 hours (1164.63 U/mg protein). After optimization an overall 4.6-fold increase in lipase production was achieved. Partial purification by (NH(4))(2)SO(4) precipitation and ion exchange chromatography resulted in 33.7% final yield. The lipase was noted to have a molecular mass of 90 kDa and optimal activity at pH 7 and 40 degrees C. Results indicated the scope for potential application of this marine fungal lipase in bioremediation. en_US
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.subject Solid-state fermentation en_US
dc.subject Microbial lipases en_US
dc.subject Extracellular lipases en_US
dc.subject Rhizopus-oryzae en_US
dc.title Lipase from marine Aspergillus awamori BTMFW032: Production, partial purification and application in oil effluent treatment en_US
dc.type Article en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search


My Account