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Improvement on citric acid production in solid-state fermentation by Aspergillus niger LPB BC mutant using citric pulp

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dc.contributor.author Rodrigues, C
dc.contributor.author de Souza Vandenberghe, L P
dc.contributor.author Teodoro, J
dc.contributor.author Pandey, A
dc.contributor.author Soccol, C R
dc.date.accessioned 2014-03-28T05:20:53Z
dc.date.available 2014-03-28T05:20:53Z
dc.date.issued 2009
dc.identifier.citation Applied Biochemistry and Biotechnology 158(1):72-87;Jul 2009 en_US
dc.identifier.issn 0273-2289
dc.identifier.uri http://ir.niist.res.in:8080/jspui/handle/123456789/1237
dc.description.abstract Citric acid (CA) production has been conducted through a careful strain selection, physical–chemical optimization and mutation. The aim of this work was to optimize the physical–chemical conditions of CA production by solid-state fermentation (SSF) using theAspergillus niger LPB BC strain, which was isolated in our laboratory. The parental and mutant strain showed a good production of CA using citric pulp (CP) as a substrate. The physical– chemical parameters were optimized and the best production was reached at 65% moisture, 30 °C and pH 5.5. The influence of the addition of commercial and alternative sugars, nitrogen sources, salts, and alcohols was also studied. The best results (445.4 g of CA/kg of CP) were obtained with sugarcane molasses and 4% methanol(v/w). The mutagenesis induction of LPB BC was performed with UV irradiation. Eleven mutant strains were tested in SSF where two mutants showed a higher CA production when compared to the parental strain. A. niger LPB B3 produced 537.6 g of CA/kg of CP on the sixth day of fermentation, while A.niger LPB B6 produced 616.5 g of CA/kg of CP on the fourth day of fermentation, representing a 19.5% and 37% gain, respectively. en_US
dc.language.iso en en_US
dc.publisher Springer en_US
dc.subject Citric acid en_US
dc.subject Citric pulp (CP) en_US
dc.subject Aspergillus niger en_US
dc.subject Solid-state fermentation en_US
dc.subject Mutation en_US
dc.title Improvement on citric acid production in solid-state fermentation by Aspergillus niger LPB BC mutant using citric pulp en_US
dc.type Article en_US

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