Gene cloning and soluble expression of Aspergillus niger phytase in E-coli cytosol via chaperone co-expression

dc.contributor.author	Ushasree, M V
dc.contributor.author	Vidya, J
dc.contributor.author	Pandey, A
dc.date.accessioned	2014-06-10T11:23:44Z
dc.date.available	2014-06-10T11:23:44Z
dc.date.issued	2014
dc.identifier.citation	Biotechnology Letters 36(1):85-91;Jan 2014	en_US
dc.identifier.issn	0141-5492
dc.identifier.uri	http://ir.niist.res.in:8080/jspui/handle/123456789/1504
dc.description.abstract	A phytase gene from Aspergillus niger was isolated and two Escherichia coli expression systems, based on T7 RNA polymerase promoter and tac promoter, were used for its recombinant expression. Co-expression of molecular chaperone, GroES/EL, aided functional cytosolic expression of the phytase in E. coli BL21 (DE3). Untagged and maltose-binding protein-tagged recombinant phytase showed an activity band of similar to 49 and 92 kDa, respectively, on a zymogram. Heterologously-expressed phytase was fractionated from endogenous E. coli phytase by (NH4)(2)SO4 precipitation. The enzyme had optimum activity at 50 A degrees C and pH 6.5.	en_US
dc.language.iso	en	en_US
dc.publisher	Springer	en_US
dc.subject	Chaperone	en_US
dc.subject	Glycosylation	en_US
dc.subject	Maltose-binding protein	en_US
dc.subject	Phytase	en_US
dc.title	Gene cloning and soluble expression of Aspergillus niger phytase in E-coli cytosol via chaperone co-expression	en_US
dc.type	Article	en_US