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Production and purification of extracellular chitinases from Penicillium aculeatum NRRL 2129 under solid-state fermentation

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dc.contributor.author Binod, P
dc.contributor.author Pusztahelyi, T
dc.contributor.author Nagy, V
dc.contributor.author Sandhya, C
dc.contributor.author Szakacs, G
dc.contributor.author Pocsi, I
dc.contributor.author Pandey, A
dc.date.accessioned 2014-10-18T06:39:58Z
dc.date.available 2014-10-18T06:39:58Z
dc.date.issued 2005
dc.identifier.citation Enzyme and Microbial Technology 36(7):880–887;May 2005 en_US
dc.identifier.issn 0141-0229
dc.identifier.uri http://ir.niist.res.in:8080/jspui/handle/123456789/1686
dc.description.abstract Fourteen Penicillium strains have been screened on wheat bran-crude chitin mixture medium for extracellular chitinase production in solid-state fermentation. Under the experimental conditions tested, Penicillium aculeatum NRRL 2129 (=ATCC 10409) was selected as the best enzyme producer. The optimum incubation period for chitinase production by the potent organism was found to be 72 h. Chromatofocusing was performed as the first step in the purification scheme, but high amount of contaminating proteins interfered with the method. Hence, ion-exchange chromatography experiments were carried-out followed by gel filtration to separate and isolate chitinase isoenzymes. Four major chitinase peaks of molecular weight 82.7, 44.6, 28.2 and 26.9 kDa were observed after gel filtration chromatography while, on SDS-PAGE, three protein bands of molecular weights 82.6, 33.9 and 29.1 kDa were identified. The purified enzyme showed optimal temperature and pH at 50 and 5.5 degrees C, respectively. en_US
dc.language.iso en en_US
dc.publisher Elsevier Science en_US
dc.subject Chitinase en_US
dc.subject Solid-state fermentation en_US
dc.subject Penicillium en_US
dc.subject Chromatography en_US
dc.title Production and purification of extracellular chitinases from Penicillium aculeatum NRRL 2129 under solid-state fermentation en_US
dc.type Article en_US


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