Xylanase production by Aspergillus niger LPB 326 in solid-state fermentation using statistical experimental designs

Abstract

Xylanase was produced by Aspergillus niger LPB 326 cultivated on lignocellulosic substrate composed by sugarcane bagasse and soybean meal in solid-state fermentation. The effects of various variables were observed and optimized by applying statistical experimental designs. The best xylanase activity was obtained in a medium containing 10 g of sugarcane bagasse and soybean meal in the ratio of 65 and 35 %, respectively, moistened to 85 % of initial water content with a nutrient salt solution composed of (in g/L): CuSO(4) 0.4, KH(2)PO(4) 1.5 and CoSO(4) 0.0012, and incubated for 4 days at 30 degrees C. Under these optimized conditions, a xylanase activity of 3099 IU/g of dry matter was obtained.