Abstract:
In the present study, media engineering was carried out to optimize various process parameters affecting
fermentation for PHB production by Comamonas sp. The optimum conditions for PHB production were
yeast extract concentration of 1%,pH6.0 and inoculum concentration of 5.5%. Under optimized conditions
the PHB yield was 0.523 g/g. The extracted polymer was characterized by FTIR and 1HNMR. Nanomatrices
and solid walled matrices were prepared using the extracted PHB. Cytotoxic studies revealed that both
solid walled and nanomatrices prepared from the extracted PHB showed almost 100% survivals of H9c2
cells. This is the first report on application of extracted PHB from Comamonas sp. as an extracellular matrix
for cell culture
