Production and characterization of PHB from a novel isolate Comamonas sp. from a dairy effluent sample and its application in cell culture

dc.contributor.author	Prabisha, T P
dc.contributor.author	Sindhu, R
dc.contributor.author	Binod, P
dc.contributor.author	Vandana, S
dc.contributor.author	Raghu, K G
dc.contributor.author	Pandey, A
dc.date.accessioned	2016-04-15T11:42:24Z
dc.date.available	2016-04-15T11:42:24Z
dc.date.issued	2015-09-15
dc.identifier.citation	Biochemical Engineering Journal 101:150-159	en_US
dc.identifier.uri	http://hdl.handle.net/123456789/2266
dc.description.abstract	In the present study, media engineering was carried out to optimize various process parameters affecting fermentation for PHB production by Comamonas sp. The optimum conditions for PHB production were yeast extract concentration of 1%,pH6.0 and inoculum concentration of 5.5%. Under optimized conditions the PHB yield was 0.523 g/g. The extracted polymer was characterized by FTIR and 1HNMR. Nanomatrices and solid walled matrices were prepared using the extracted PHB. Cytotoxic studies revealed that both solid walled and nanomatrices prepared from the extracted PHB showed almost 100% survivals of H9c2 cells. This is the first report on application of extracted PHB from Comamonas sp. as an extracellular matrix for cell culture	en_US
dc.language.iso	en	en_US
dc.publisher	Elsevier	en_US
dc.subject	Poly-3-hydroxybutyrate Comamonas sp; Fermentation ;Cell culture ;Matrices Optimization	en_US
dc.title	Production and characterization of PHB from a novel isolate Comamonas sp. from a dairy effluent sample and its application in cell culture	en_US
dc.type	Article	en_US