Abstract:
This studydescribeshow Achromobacterdenitrificans strainSP1,anovelisolatefromheavilyplastics-
contaminated sewagesludgeefficiently consumedthehazardousplasticizer,di(2-ethylhexyl)phthalate
(DEHP) ascarbonsourcesupplementedinasimplebasalsaltmedium(BSM).Responsesurfacemeth-
odology wasemployedforthestatisticaloptimizationoftheprocessparameterssuchastemperature
(32 °C), agitation(200rpm),DEHPconcentration(10mM),time(72h)andpH(8.0).Attheseoptimized
conditions, experimentallyobservedDEHPdegradationwas63%,whilethepredictedvaluewas59.2%;
and thecorrelationcoefficient betweenthemwas0.998, i.e., highlysignificant and fit tothepredicted
model. EmployingGC–MS analysis,thedegradationpathwaywaspartiallydeducedwithintermediates
such asmono(2-ethylhexyl)phthalateand2-ethylhexanol.Briefly,this first reportdescribes A.deni-
trificans strainSP1asahighlyefficient bacteriumforcompletelyremediatingthehazardousDEHP
(10mM)in96hinBSM(50%consumedin60h),whichoffersgreatpotentialsforefficiently cleaningthe
DEHP-contaminatedenvironmentssuchassoil,sedimentsandwateruponitsdeployment
