Abstract:
Nanotechnology for the treatment and diagnosis has been emerging recently as a potential area of
research and development. In the present study, selenium incorporated guar gum nanoparticles have
been prepared by nanoprecipitation and characterized by transmission electron microscopy and particle
size analysis. The nanoparticles were screened for antioxidant potential (metal chelation, total reducing
power and hydroxyl radical scavenging activity) and were evaluated against the cell line based cardiac
ischemia/reperfusion model with special emphasis on oxidative stress and mitochondrial parameters.
The cell based cardiac ischemia model was employed using H9c2 cell lines. Investigations revealed that
there was a significant alteration (Pr0.05) in the innate antioxidant status (glutathionek, glutathione
peroxidasek, thioredoxin reductasek, superoxide dismutasek, catalasek, lipid peroxidationm, protein
carbonylm, xanthine oxidasemand caspase 3 activitym), mitochondrial functions (reactive oxygen species
generation, membrane potential, and pore opening) and calcium homeostasis (calcium ATPase and
intracellular calcium overload) during both ischemia and reperfusion. For comparative evaluation,
selenium, guar gum and selenium incorporated guar gum nanoparticles were evaluated for their
protective properties against ischemia/reperfusion. The study reveals that selenium incorporated guar
gum nanoparticles were better at protecting the cells from ischemia/reperfusion compared to selenium
and guar gum nanoparticles. The potent antioxidant capability shown by the sample inin vitro assays
may be the biochemical basis of its better biological activity. Further, the nanodimensions of the particle
may be the additional factor responsible for its better effect.
