Abstract:
This study deals with the preparation and characterization of selenium incorporated guar gum nanoparticle (SGG), and its
effect on H9c2 cardiomyoblast. Herein, nanoprecipitation techniques had been employed for the preparation of SGG
nanoparticle. The prepared nanoparticle had been subjected to various types of analytical techniques like transmission
electron microscopy (TEM), X-ray diffraction (XRD) and particle size analysis to confirm the characteristics of nanoparticle as
well as for selenium incorporation. Physical characterization of nanoparticle showed that the size of nanoparticles increase
upto ,69–173 nm upon selenium incorporation from ,41–132 nm. Then the prepared nanoparticles were evaluated for its
effect on H9c2 cells. In this regard, the effect of nanoparticle on various vital parameters of H9c2 cells was studied.
Parameters like cell viability, uptake of selenium incorporated guar gum nanoparticle by the cells, effect of SGG on DNA
integrity, apoptosis, reactive oxygen species generation, alteration in transmembrane potential of mitochondria and
cytoskeletal integrity had been investigated. Viability results showed that up to 25 nM of SGG was safe (10.31%) but beyond
that it induces cytotoxicity. Cellular uptake of selenium showed that cell permeability for SGG is significantly high compared
to normal selenium (7.2 nM of selenium for 25 nM SGG compared with 5.2 nM selenium for 25 nM sodium selenite). There
was no apoptosis with SGG and also it protects DNA from hydroxyl radical induced breakage. Likewise no adverse effect on
mitochondria and cytoskeleton was observed for 25 nM of SGG. Overall results reveal that SGG is highly suitable for
biomedical research application.
