Abstract:
Bioactivity directed fractionation and isolation of active principles
from the pericarp of Annona squamosa yielded three kauranes namely,
(-)-ent-kaur-17-acetoxy, 19-oic acid, 16α, 17-dihydroxy-ent-kauran-19-
oic acid and (-)-ent-kaur-17, 19-dioic acid. These compounds were tested
against two bacteria, Staphylococus aureus (MTCC 737, S.N. Saxena,
Staphlococcus Institute of Microbial Technology) and Escherichia. coli
(MTCC 2991, N. Goel, Enterobacteriaceae, Institute of Microbial
Technology). 16α, 17-Dihydroxy-ent-kauran-19-oic acid and (-)-entkaur-
17, 19-dioic acid at 100 μg and 500μg, respectively, showed more
activity towards Escherichia. coli than the antibiotic, ampicillin at 500
μg and these kaurenes do not show promising activity towards Staphylococcus
aureus, when compared with the standard drug, gentamycin.
Here, we report the bioactivity directed fractionation, isolation and identification
of the active principles by IR, NMR, MS and by the chemical
conversions of the active compounds
