Abstract:
A bioactive compound was purified from the
culture medium of a new strain of Lactococcus BSN307 by
solvent extraction followed by chromatographic techniques.
This bioactive compound was identified to belong
to phenazine class of compounds by MS, NMR and FTIR.
The phenazine compound showed antifungal activity
against Aspergillus niger, Penicillium chrysogenum as well
as Fusarium oxysporum by disc diffusion assay in addition
to antioxidant potential as demonstrated by DPPH scavenging
assay. The compound demonstrated selective
cytotoxicity against cancer cell lines HeLa and MCF-7
where IC50 was achieved with 20 and 24 lg/mL respectively.
At the same time no cytotoxicity was occurred in
normal H9c2 cells. The bioactive found to be inhibitory to
both leucine and proline aminopeptidases and thus revealed
its potential as metalloenzyme inhibitor. This study, for the
first time reports the production of phenazine class of
compounds by lactic acid bacteria.
