Abstract:
The present study evaluated antidiabetic potential of fractions of Syzygium cumini seeds and identified
major polyphenolic compounds in them. Potential a-glucosidase and a-amylase inhibition
(IC50 1.7 and 7.62 lg/ml, respectively) was demonstrated by 70% methanol fraction while significant
dipeptidyl peptidase-IV inhibition (88.1%) was demonstrated by methanol fraction. A fourfold
increase in glucose uptake in L6 cells following the pretreatment of 70% methanol fraction
(26.9%) further confirmed the antidiabetic potential of S. cumini seeds. The total phenolic (906 mg
GAE/g dry weight) and flavonoid content (233 mg QE/g dry weight) were highest in 70% methanol
fraction. Phenolic profiling of fractions through HPLC showed the presence of quercetin,
cinnamic acid, syringic acid, ferulic acid, ellagic acid, and gallic acid, which was further confirmed
through LC–Q-ToF (MS/MS). Major phenolics identified were docked with studied enzymes and
analyzed. All the results suggest that S. cumini seed fractions have significant potential in the management
of diabetes.
