dc.contributor.author |
Ranjan, P |
|
dc.contributor.author |
Pandey, A |
|
dc.contributor.author |
Binod, P |
|
dc.date.accessioned |
2018-06-22T09:27:10Z |
|
dc.date.available |
2018-06-22T09:27:10Z |
|
dc.date.issued |
2017-09 |
|
dc.identifier.citation |
Journal of Basic Microbiology, 57(9):762–769 |
en_US |
dc.identifier.uri |
http://10.10.100.66:8080/xmlui/handle/123456789/3141 |
|
dc.description.abstract |
Chiral intermediates have wide application and high demand in pharmaceutical, agricultural, and other biotechnological industries for the preparation of bulk drug substances or fine chemicals. (S)-1-(1-napthyl) ethanol is an important synthetic intermediate of mevinic acid analog and a potential inhibitor of 3-hydroxy methyl glutaryl coenzyme A reductase enzymes which is rate limiting for cholesterol synthesis. The present study focuses on the resolution of (RS)-1-(1-napthyl) ethanol using whole cell biotransformation approach. The screening of microbial strains for the specific conversion were performed by the enrichment techniques using (RS)-1- (1-napthyl) ethanol. Evaluation of resolution, i.e., the enantioselective conversion of (R)-1-(1-napthyl) ethanol into 1-acetonapthone and production of (S)-1-(1-napthyl) ethanol with high purity were carried out. Among the isolates, a novel strain Bacillus cereus WG3 was found to be potent for the resolution and conversion of (S)-1-(1- napthyl) ethanol. This strain showed 86% conversion of (R)-1-(1-napthyl) ethanol
and 95% yield of S-1-(1-napthyl) ethanol with 80% ee after 24 h. Further, the optimization of biotransformation reactions was carried out and the optimal parameters were found to be pH 7.0 and temperature 30 °C. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Wiley |
en_US |
dc.subject |
chiral resolution |
en_US |
dc.subject |
enantio-selective |
en_US |
dc.subject |
biotransformation |
en_US |
dc.subject |
chiral molecule |
en_US |
dc.subject |
(S)-1-(1-napthyl) ethanol |
en_US |
dc.title |
Resolution of Enantiopure (S)-1-(1-Napthyl) Ethanol from Racemic Mixture by a Novel Bacillus Cereus Isolate |
en_US |
dc.type |
Article |
en_US |