Abstract:
A simple and reproducible method for the qualitative and quantitative analysis of free mono- (fructose, glucose) and disaccharides (sucrose) using a high performance liquid chromatography (HPLC) with refractive index (RI) detector and a NH2 column was developed for matrices rich in monosaccharides (honey) and disaccharides (palm sap, palm syrup, sugarcane jaggery, palm jaggery). The developed HPLC method was validated in terms of their linearity, limit of detection and quantification, precision and accuracy. Method validation studies showed that 85:15 (v/v) acetonitrile/water (CH3CN:H2O) was suitable for matrix containing monosaccharides and 65:35
(v/v) (CH3CN: H2O) was suitable for matrix containing disaccharides. The method showed good linearity with determination coefficients exceeding 0.99. Recovery studies indicated that 65% (CH3CN:H2O) system was suitable for matrix rich in sucrose (e.g. jaggery, syrup, etc.).
