Domibacillus Mangrovi Sp. Nov. and Domibacillus Epiphyticus Sp. Nov., Isolated from Marine Habitats of the Central West Coast of India

Abstract

While studying culturable bacterial diversity in different marine habitats of the central west coast of India, two novel Gramstain- positive, strictly aerobic, motile, endospore-forming and rod-shaped bacterial strains designated as SAOS 44T and SAB 38T were isolated from mangrove sediment soil and the surface of a macroalga, respectively. The strains were taxonomically characterized by using a polyphasic approach and genomic methods. The phylogenetic analysis based on 16S rRNA gene sequencing placed the strains firmly in the genus Domibacillus and were most closely related to Domibacillus antri KCTC 33636T. The predominant fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol in addition to an aminophosphoglycolipid. MK-6 was the only respiratory quinone. The range of values of digital DNA–DNA hybridization (19.2–24.9 %) and the ortho-average nucleotide identity (74.1– 81.4 %) among strains SAOS 44T, SAB 38T and other Domibacillus species clearly supports their status as a distinct and novel species for which the names Domibacillusmangrovisp. nov. SAOS 44T (=DSM 100930T=KCTC 33820T) and Domibacillusepiphyticussp. nov. SAB 38T (=DSM 100929T=KCTC 33830T) are proposed, respectively.