Abstract:
This study presents production of alpha amylase by Apergillus oryzae in solid-state fementation using 14 agro-industrial wastes as substrate. Enzyme production was growth associated and maximum titers (15095 U/gds) were obtained after 72 h when incubated at 30°C on wheat bran (initial moisture content, 60%, initial medium pH,5). Enzyme titers increased significantly when the solid medium was supplemented with additional N (Sodium nitrate) and C (Starch) sources.