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Optimization of cellulose production from Apergillus flavipes by submerged and solid state fermentation

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dc.contributor.author Dina, S
dc.contributor.author Thankamani, V T
dc.contributor.author Emilia Abraham, T
dc.date.accessioned 2023-05-08T09:51:04Z
dc.date.available 2023-05-08T09:51:04Z
dc.date.issued 2023-01-19
dc.identifier.citation Journal of microbiology, biotechnology and food sciences; 12(5) en_US
dc.identifier.uri https://office2.jmbfs.org/index.php/JMBFS/article/view/4754
dc.identifier.uri http://localhost:8080/xmlui/handle/123456789/4479
dc.description.abstract Cellulase are important class of enzymes that has potential applications in textile, biofuel, food and feed industry. Fungi such as Aspergillus, Trichoderma are reliable sources of cellulase production. Optimisation of multiple parameters is required for efficient cellulase production in high titers in both SmF and SSF. Carbon sources such as lactose, maltose, cellulose, nitrogen sources including ammonium dihydrogen phosphate and beef extract enhance cellulase activities. Thiamine, Nicotinic acid and all metal chlorides except CuCl2 enhanced cellulase production. Based on the one factor at a time model, optimum medium was designed which produced cellulase of 0.767 FPU/ml by SmF. Maximum CMCase, FPase activity and protein were produced at pH 4.5 at 150 rpm and with a working volume ratio (wvr) of 0.2. The production of cellulase was 4.6 fold higher than the un-optimized media. SSF is an ideal fermentation method for cellulase production by fungi. Maximum CMCase, FPase and pNPG activities were recorded at 50% moisture level, pH 5.0 and below 40 °C. Wheat bran acted as a best carbon source with higher FPase, pNPG and CMCase (5.6 IU/g, 17 IU/g and 14 IU/g carbon source) activities. A. flavipes thus acts a perfect fungal source for cellulase production both by SmF and SSF. en_US
dc.language.iso en en_US
dc.publisher Faculty of Biotechnology and Food Sciences en_US
dc.subject cellulase en_US
dc.subject solid state fermentation en_US
dc.subject submerged fermentation en_US
dc.subject A. flavipes en_US
dc.subject endoglucanase en_US
dc.subject exoglucanase en_US
dc.subject β-glucosidase en_US
dc.title Optimization of cellulose production from Apergillus flavipes by submerged and solid state fermentation en_US
dc.type Article en_US


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  • 2023
    Research articles authored by NIIST researchers published in 2023

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