Abstract:
Understanding and controlling the interactions between nanoparticles and living
cells are of great importance in the diagnosis and therapeutic applications of
nanosized materials. This article describes the synthesis, characterisation and
interactions of gold nanoparticles (AuNPs) with different in vitro and in vivo
experimental models. Preliminary cytotoxicity studies were carried out in tumour ascites (Dalton’s lymphoma ascites and Ehrlich’s ascites carcinoma) and normal
peritoneal cells for different concentrations and it was found that AuNPs did not cause any cell death or morphological changes even at 100mM concentrations.The tissue distribution and toxicity of intravenously administered AuNPs were investigated in mice because of the fundamental importance of obtaining
information about the localisation and biocompatibility of this material. Tissue
distribution of AuNPs was studied in normal as well as skin tumour-induced Swiss albino mice using inductively coupled plasma-atomic emission spectrophotometer and transmission electron microscopy. Acute cytotoxicity and
histopathology were carried out for toxicity evaluation in AuNP-injected normal mice and found that AuNPs did not cause any significant metabolic change. The health and behaviour of animals were normal throughout the study.The biocompatibility assessment of AuNPs both in vitro and in vivo confirmed
that triethylene glycol-functionalised AuNPs are compatible to the biological system and can be used as a safe material for various biological applications
