Production and partial purification of alpha-amylase from a novel isolate streptomyces gulbargensis

Dastager, S G; Agasar, D; Pandey, A

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dc.contributor.author	Dastager, S G
dc.contributor.author	Agasar, D
dc.contributor.author	Pandey, A
dc.date.accessioned	2013-11-26T09:23:01Z
dc.date.available	2013-11-26T09:23:01Z
dc.date.issued	2009
dc.identifier.citation	Journal of Industrial Microbiology & Biotechnology 36(2):189-194;Feb 2009	en_US
dc.identifier.issn	1367-5435
dc.identifier.uri	http://ir.niist.res.in:8080/jspui/handle/123456789/841
dc.description.abstract	Extracellular amylase production by a newly isolated alkali-thermotolerant strain Streptomyces gulbargensis DAS 131 was optimized and characterized. The highest amylase production was achieved by growing S. gulbargensis DAS 131 in media with 1% starch. Strain exhibited maximal activity at pH 9.0 and 45A degrees C and relatively stable in alkaline conditions (pH 11). Starch and peptone were found to be the good source of carbon and nitrogen with a yield of 2,216.6 and 2,156.1 U, respectively. Maltose and maltotriose were the main end products of starch hydrolysis, indicating alpha-amylase activity. SDS-PAGE analysis revealed a monomeric form with a molecular weight of 55 kDa.	en_US
dc.language.iso	en	en_US
dc.publisher	Springer	en_US
dc.subject	Streptomyces	en_US
dc.subject	Amylolytic enzymes	en_US
dc.subject	Purification	en_US
dc.subject	pH and temperature effect	en_US
dc.subject	Enzymatic properties	en_US
dc.subject	Alkaliphilic bacillus isolate	en_US
dc.subject	Extracellular amylase	en_US
dc.title	Production and partial purification of alpha-amylase from a novel isolate streptomyces gulbargensis	en_US
dc.type	Article	en_US